CAPILLARY ISOTACHOPHORETIC ANALYTE FOCUSING FOR CAPILLARY ELECTROPHORESIS WITH MASS-SPECTROMETRIC DETECTION USING ELECTROSPRAY IONIZATION

被引：40

作者：

REINHOUD, NJ ^{[1
]}

TINKE, AP ^{[1
]}

TJADEN, UR ^{[1
]}

NIESSEN, WMA ^{[1
]}

VANDERGREEF, J ^{[1
]}

机构：

[1] LEIDEN UNIV, CTR BIOPHARMACEUT SCI, DIV ANALYT CHEM, POB 9502, 2300 RA LEIDEN, NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 627卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)87206-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An improvement in detectability in capillary electrophoresis-mass spectrometry (CE-MS) was realized using isotachophoretic analyte focusing. The practical approach is described for the on-line coupling of capillary isotachophoresis-capillary electrophoresis-mass spectrometry (ITP-CE-MS) using an electrospray interface. An equation was derived for the calculation of the splitting ratio of ITP zones into the CE system. The applicability of analyte focusing is demonstrated for the analysis of a mixture of anthracyclines. Initial experiments showed a 200-fold improvement in concentration detection limit for ITP-CE-MS compared with CE-MS.

引用

页码：263 / 271

页数：9

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