PRODUCTION OF RECOMBINANT CYTOCHROME C-551 IN A PSEUDOMONAS-AERUGINOSA MUTANT STRAIN

被引：11

作者：

ARAI, H ^{[1
]}

ZHANG, Y ^{[1
]}

SAMBONGI, Y ^{[1
]}

IGARASHI, Y ^{[1
]}

KODAMA, T ^{[1
]}

机构：

[1] UNIV TOKYO,DEPT BIOTECHNOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1995年 / 79卷 / 05期

关键词：

CYTOCHROME C; SIGNAL PEPTIDE; PSEUDOMONAS AERUGINOSA;

D O I：

10.1016/0922-338X(95)91267-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A mutant of Pseudomonas aeruginosa lacking the cytochrome c-551 gene (nirM) was constructed by marker exchange mutagenesis. When the nirM gene was introduced in this strain using a multi-copy expression vector, pHA10, recombinant cytochrome c-551 was produced as a hole-form under aerobic conditions, whereas the expression was not observed when the nirM gene specifically lacking the signal sequence for membrane translocation was used.

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收藏

页码：489 / 492

页数：4

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