IDENTIFICATION OF MURINE HOMOLOGS OF THE DROSOPHILA SON OF SEVENLESS GENE - POTENTIAL ACTIVATORS OF RAS

被引:290
作者
BOWTELL, D
FU, P
SIMON, M
SENIOR, P
机构
[1] UNIV CALIF BERKELEY,HOWARD HUGHES MED INST,BERKELEY,CA 94720
[2] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,BERKELEY,CA 94720
关键词
TYROSINE KINASE; RECEPTOR; SIGNAL TRANSDUCTION; RAS;
D O I
10.1073/pnas.89.14.6511
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Several findings suggest that signals from tyrosine kinases are transduced, at least in part, through ras proteins. These findings include (i) blockage of the transforming activity of constitutively active tyrosine kinases by inhibiting ras function and (ii) genetic screens in Caenorhabditis elegans and in Drosophila that identified ras genes as downstream effectors of tyrosine kinases. The recently isolated Drosophila gene Son of sevenless (Sos) is postulated to act as a positive regulatory link between tyrosine kinase and ras proteins by catalyzing exchange of GDP for GTP on ras protein. Such exchange proteins have been reported in extracts of mammalian cells but have not been previously characterized at a molecular level. As Sos appears to function in this role in Drosophila, we sought to isolate a vertebrate counterpart(s). We have characterized two widely expressed murine genes with a high degree of homology to Sos. Hybridization with human DNA and RNA indicates a high degree of conservation of these genes in other vertebrates.
引用
收藏
页码:6511 / 6515
页数:5
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