MOLECULAR-CLONING OF THE HUMAN KIDNEY DIFFERENTIATION ANTIGEN GP160 - HUMAN AMINOPEPTIDASE-A

被引:118
作者
NANUS, DM
ENGELSTEIN, D
GASTL, GA
GLUCK, L
VIDAL, MJ
MORRISON, M
FINSTAD, CL
BANDER, NH
ALBINO, AP
机构
[1] MEM SLOAN KETTERING CANC CTR, LUDWIG INST CANC RES, NEW YORK UNIT, NEW YORK, NY 10021 USA
[2] MEM SLOAN KETTERING CANC CTR, SOLID TUMOR IMMUNOL LAB, NEW YORK, NY 10021 USA
[3] MEM SLOAN KETTERING CANC CTR, HUMAN CANC IMMUNOL LAB, NEW YORK, NY 10021 USA
[4] MEM SLOAN KETTERING CANC CTR, DEPT MED, DIV SOLID TUMOR ONCOL, GENITOURINARY SERV, NEW YORK, NY 10021 USA
[5] CORNELL UNIV, MED CTR, NEW YORK HOSP, DEPT SURG, DIV UROL, NEW YORK, NY 10021 USA
关键词
CDNA CLONING; RENAL CANCER; ECTOPEPTIDASES; ANGIOTENSINS;
D O I
10.1073/pnas.90.15.7069
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
gp160 is a cell surface differentiation-related glycoprotein of 160 kDa expressed by epithelial cells of the glomerulus and proximal tubule cells of the human nephron but only by a subset of renal cell carcinomas (RCCs). We have reported that gp160 expression correlates with the resistance of cultured RCCs to the antiproliferative effects of alpha interferon, while lack of expression correlates with sensitivity to alpha interferon. In this study, we have purified gp160 protein, obtained partial sequences of random peptides, and isolated a full-length cDNA. The gp160 cDNA possesses 78% homology to the murine BP-1/6C3 antigen, a B-lymphocyte differentiation protein that exhibits aminopeptidase A (APA; EC 3.4.11.7) activity. Enzymatic assays on human RCC cell lines indicated a 100% concordance between APA activity and gp160 expression. APA activity of gp160-expressing RCC cells was increased or decreased by a panel of APA activators or inhibitors, respectively. Furthermore, anti-gp160 monoclonal antibodies immunoprecipitate APA activity from RCC cell lysates and selectively deplete APA activity from RCC cell extracts. These data indicate that the gp160 human kidney/RCC glycoprotein is human APA.
引用
收藏
页码:7069 / 7073
页数:5
相关论文
共 48 条
  • [21] THE ORIGIN OF MATRIX METALLOPROTEINASES AND THEIR FAMILIAL RELATIONSHIPS
    MURPHY, GJP
    MURPHY, G
    REYNOLDS, JJ
    [J]. FEBS LETTERS, 1991, 289 (01): : 4 - 7
  • [23] NANUS DM, 1990, CANCER RES, V50, P4190
  • [24] INFREQUENT RAS ONCOGENE POINT MUTATIONS IN RENAL-CELL CARCINOMA
    NANUS, DM
    MENTLE, IR
    MOTZER, RJ
    BANDER, NH
    ALBINO, AP
    [J]. JOURNAL OF UROLOGY, 1990, 143 (01) : 175 - 178
  • [25] TRANSFORMATION OF HUMAN-KIDNEY PROXIMAL TUBULE CELLS BY RAS-CONTAINING RETROVIRUSES - IMPLICATIONS FOR TUMOR PROGRESSION
    NANUS, DM
    EBRAHIM, SAD
    BANDER, NH
    REAL, FX
    PFEFFER, LM
    SHAPIRO, JR
    ALBINO, AP
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 169 (03) : 953 - 972
  • [26] NISHIKAWA BK, 1989, BIOTECHNIQUES, V7, P730
  • [27] AMINOPEPTIDASE-A IN HUMAN PLACENTA AND PREGNANT SERUM
    OKUYAMA, T
    ISHIURA, S
    NOJIMA, M
    TSUKAHARA, T
    YANAGIDA, M
    SUGITA, H
    [J]. CLINICA CHIMICA ACTA, 1991, 196 (2-3) : 207 - 215
  • [28] COMPLETE AMINO-ACID SEQUENCE OF HUMAN INTESTINAL AMINOPEPTIDASE-N AS DEDUCED FROM CLONED CDNA
    OLSEN, J
    COWELL, GM
    KONIGSHOFER, E
    DANIELSEN, EM
    MOLLER, J
    LAUSTSEN, L
    HANSEN, OC
    WELINDER, KG
    ENGBERG, J
    HUNZIKER, W
    SPIESS, M
    SJOSTROM, H
    NOREN, O
    [J]. FEBS LETTERS, 1988, 238 (02) : 307 - 314
  • [29] IMPROVED TOOLS FOR BIOLOGICAL SEQUENCE COMPARISON
    PEARSON, WR
    LIPMAN, DJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (08) : 2444 - 2448
  • [30] RAMAKRISHNAN L, 1990, J IMMUNOL, V145, P1603