SUBSTRATE-DERIVED 2-PROTONIC-STATE ELECTROPHILES AS SENSITIVE KINETIC SPECIFICITY PROBES FOR CYSTEINE PROTEINASES - ACTIVATION OF 2-PYRIDYL DISULFIDES BY HYDROGEN-BONDING

被引：34

作者：

BROCKLEHURST, K ^{[1
]}

KOWLESSUR, D ^{[1
]}

ODRISCOLL, M ^{[1
]}

PATEL, G ^{[1
]}

QUENBY, S ^{[1
]}

SALIH, E ^{[1
]}

TEMPLETON, W ^{[1
]}

THOMAS, EW ^{[1
]}

WILLENBROCK, F ^{[1
]}

机构：

[1] UNIV SALFORD, DEPT BIOL SCI, SALFORD M5 4WT, LANCS, ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1987年 / 244卷 / 01期

关键词：

D O I：

10.1042/bj2440173

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：173 / 181

页数：9

共 46 条

[1] ALBERT A, 1963, PHYS METHODS HETEROC, V1, P1
[2] CHARACTERIZATION OF PAPAYA PEPTIDASE-A AS A CYSTEINE PROTEINASE OF CARICA-PAPAYA L WITH ACTIVE-CENTER PROPERTIES THAT DIFFER FROM THOSE OF PAPAIN BY USING 2,2'-DIPYRIDYL DISULFIDE AND 4-CHLORO-7-NITROBENZOFURAZAN AS REACTIVITY PROBES - USE OF 2-PROTONIC-STATE ELECTROPHILES IN THE IDENTIFICATION OF CATALYTIC-SITE THIOL-GROUPS
BAINES, BS
BROCKLEHURST, K
[J]. BIOCHEMICAL JOURNAL, 1982, 205 (01) : 205 - 211
[3] CHYMOPAPAIN-A - PURIFICATION AND INVESTIGATION BY COVALENT CHROMATOGRAPHY AND CHARACTERIZATION BY 2-PROTONIC-STATE REACTIVITY-PROBE KINETICS, STEADY-STATE KINETICS AND RESONANCE RAMAN-SPECTROSCOPY OF SOME DITHIOACYL DERIVATIVES
BAINES, BS
BROCKLEHURST, K
CAREY, PR
JARVIS, M
SALIH, E
STORER, AC
[J]. BIOCHEMICAL JOURNAL, 1986, 233 (01) : 119 - 129
[4] NECESSARY MODIFICATION TO THE PREPARATION OF PAPAIN FROM ANY HIGH-QUALITY LATEX OF CARICA-PAPAYA AND EVIDENCE FOR THE STRUCTURAL INTEGRITY OF THE ENZYME PRODUCED BY TRADITIONAL METHODS
BAINES, BS
BROCKLEHURST, K
[J]. BIOCHEMICAL JOURNAL, 1979, 177 (02) : 541 - 548
[5] THE SPECIFICITY OF ACTINIDIN AND ITS RELATIONSHIP TO THE STRUCTURE OF THE ENZYME
BAKER, EN
BOLAND, MJ
CALDER, PC
HARDMAN, MJ
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 616 (01) : 30 - 34
[6] MAPPING ACTIVE SITE OF PAPAIN WITH AID OF PEPTIDE SUBSTRATES AND INHIBITORS
BERGER, A
SCHECHTER, I
[J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1970, 257 (813) : 249 - +
[7] EVIDENCE FROM 2-PROTONIC-STATE REACTIVITY PROBE KINETICS THAT CHYMOPAPAIN IN FRESH NONFRUIT LATEX OF CARICA-PAPAYA CONSISTS OF MULTIPLE FORMS OF CHYMOPAPAIN-A - THE VALUE OF CATALYTIC SITE CHARACTERISTICS IN THE IDENTIFICATION, CLASSIFICATION, AND CHARACTERIZATION OF THE PAPAYA CYSTEINE PROTEINASES PAPAIN, THE CHYMOPAPAINS, AND PAPAYA PROTEINASE-OMEGAC
BROCKLEHURST, K
MCKEE, R
SALIH, E
SMITH, H
[J]. JOURNAL OF PROTEIN CHEMISTRY, 1985, 4 (02): : 103 - 127
[8] PH-DEPENDENCE OF STEADY-STATE RATE OF A 2-STEP ENZYMIC REACTION
BROCKLEHURST, K
DIXON, HBF
[J]. BIOCHEMICAL JOURNAL, 1976, 155 (01) : 61 - 70
[9] EQUILIBRIUM ASSUMPTION IS VALID FOR THE KINETIC TREATMENT OF MOST TIME-DEPENDENT PROTEIN-MODIFICATION REACTIONS
BROCKLEHURST, K
[J]. BIOCHEMICAL JOURNAL, 1979, 181 (03) : 775 - 778
[10] DIFFERENCES IN THE INTERACTIONS OF THE CATALYTIC GROUPS OF THE ACTIVE-CENTERS OF ACTINIDIN AND PAPAIN - RAPID PURIFICATION OF FULLY ACTIVE ACTINIDIN BY COVALENT CHROMATOGRAPHY AND CHARACTERIZATION OF ITS ACTIVE-CENTER BY USE OF 2-PROTONIC-STATE REACTIVITY PROBES
BROCKLEHURST, K
BAINES, BS
MALTHOUSE, JPG
[J]. BIOCHEMICAL JOURNAL, 1981, 197 (03) : 739 - 746

← 1 2 3 4 5 →