THE ISOMERIZATION OF D-GLUCOSE INTO D-FRUCTOSE CATALYZED BY WHOLE-CELL IMMOBILIZED GLUCOSE-ISOMERASE - THE DEPENDENCE OF THE INTRINSIC RATE OF REACTION ON SUBSTRATE CONCENTRATION, PH, AND TEMPERATURE

The relation between the intrinsic rate of the glucose-fructose isomerization catalyzed by whole-cell immobilized glucose isomerase and the substrate concentration can be described with the kinetic model The numerical values of k1, k-1, k2, and k-2 have been determined from low-conversion experiments starting from pure glucose or fructose solutions, and are presented as a function of pH and temperature. The difference between the overall chemical reaction rate determined in high-conversion experiments and that calculated from the individual k1, k-1, k2, and k-2 values is less than 10%. © 1981 John Wiley & Sons, Inc.