EFFECTS OF DEFOCUS AND PRIMARY SPHERICAL-ABERRATION ON 3-DIMENSIONAL COHERENT TRANSFER-FUNCTIONS IN CONFOCAL MICROSCOPES

被引：17

作者：

GU, M

SHEPPARD, CJR

机构：

[1] Department of Physical Optics, University of Sydney, Sydney, NSW

来源：

APPLIED OPTICS | 1992年 / 31卷 / 14期

关键词：

D O I：

10.1364/AO.31.002541

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

For both reflection- and transmission-mode confocal scanning microscope systems, three-dimensional coherent transfer functions are investigated by considering the effects of defocus and primary spherical aberration. We numerically calculate the three-dimensional coherent transfer functions for various amounts of aberration and show that three-dimensional confocal imaging is strongly degraded if the amount of aberration is larger than a quarter wavelength. The compensation of the primary spherical aberration by introducing defocus is also discussed.

引用

页码：2541 / 2549

页数：9

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