IDENTIFICATION OF P42 MITOGEN-ACTIVATED PROTEIN-KINASE AS A TYROSINE KINASE SUBSTRATE ACTIVATED BY MAXIMAL ELECTROCONVULSIVE SHOCK IN HIPPOCAMPUS

被引:75
作者
BARABAN, JM
FIORE, RS
SANGHERA, JS
PADDON, HB
PELECH, SL
机构
[1] JOHNS HOPKINS UNIV,SCH MED,DEPT PSYCHIAT & BEHAV SCI,BALTIMORE,MD 21205
[2] UNIV BRITISH COLUMBIA,BIOMED RES LAB,VANCOUVER V6T 1W5,BC,CANADA
[3] UNIV BRITISH COLUMBIA,DEPT MED,VANCOUVER V6T 1W5,BC,CANADA
关键词
PROTEIN TYROSINE PHOSPHORYLATION; EXTRACELLULAR SIGNAL-REGULATED KINASES; SEIZURES;
D O I
10.1111/j.1471-4159.1993.tb05855.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recent studies have demonstrated that administration of an electroconvulsive shock produces a rapid and transient increase in tyrosyl phosphorylation of a approximately 40-kDa Protein in rat brain. Initial characterization of this protein's chromatographic properties indicated that it might be a member of a recently identified family of kinases, referred to as mitogen-activated protein (MAP) kinases, that are activated by tyrosyl phosphorylation. In the present study, we have used MAP kinase antisera to assess the identity of this protein. We have found that the approximately 40-kDa phosphotyrosine-containing protein comigrates with p42 MAP kinase (p42mapk) and not with two other 44-kDa MAP kinase family members detected by these antisera. Western blots of proteins immunoprecipitated with MAP kinase antibodies confirm that p42mapk displays increased tyrosyl phosphorylation after an electroconvulsive stimulus. Chromatographic separation of hippocampal extracts indicates that MAP kinase activity elutes in parallel with p42mapk. Accordingly, these studies identify p42mapk as a tyrosyl kinase substrate that is activated by this stimulus and suggest that this form of MAP kinase may be selectively regulated by neuronal stimulation.
引用
收藏
页码:330 / 336
页数:7
相关论文
共 49 条
[31]   ACTIVATION OF MYELIN BASIC-PROTEIN KINASES DURING ECHINODERM OOCYTE MATURATION AND EGG FERTILIZATION [J].
PELECH, SL ;
TOMBES, RM ;
MEIJER, L ;
KREBS, EG .
DEVELOPMENTAL BIOLOGY, 1988, 130 (01) :28-36
[32]   MITOGEN-ACTIVATED PROTEIN-KINASES - VERSATILE TRANSDUCERS FOR CELL SIGNALING [J].
PELECH, SL ;
SANGHERA, JS .
TRENDS IN BIOCHEMICAL SCIENCES, 1992, 17 (06) :233-238
[33]   TYROSINE PHOSPHORYLATION AND ACTIVATION OF HOMOLOGOUS PROTEIN-KINASES DURING OOCYTE MATURATION AND MITOGENIC ACTIVATION OF FIBROBLASTS [J].
POSADA, J ;
SANGHERA, J ;
PELECH, S ;
AEBERSOLD, R ;
COOPER, JA .
MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (05) :2517-2528
[34]   REQUIREMENTS FOR PHOSPHORYLATION OF MAP KINASE DURING MEIOSIS IN XENOPUS OOCYTES [J].
POSADA, J ;
COOPER, JA .
SCIENCE, 1992, 255 (5041) :212-215
[35]   PHOSPHORYLATION OF C-JUN MEDIATED BY MAP KINASES [J].
PULVERER, BJ ;
KYRIAKIS, JM ;
AVRUCH, J ;
NIKOLAKAKI, E ;
WOODGETT, JR .
NATURE, 1991, 353 (6345) :670-674
[36]   RAPID STIMULATION BY INSULIN OF A SERINE THREONINE KINASE IN 3T3-L1 ADIPOCYTES THAT PHOSPHORYLATES MICROTUBULE-ASSOCIATED PROTEIN-2 INVITRO [J].
RAY, LB ;
STURGILL, TW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (06) :1502-1506
[37]  
RAY LB, 1988, J BIOL CHEM, V263, P12721
[38]  
SANGHERA J, 1992, BIOCHIM BIOPHYS ACTA, V1095, P153
[39]  
SANGHERA JS, 1991, J BIOL CHEM, V266, P6700
[40]  
SANGHERA JS, 1990, J BIOL CHEM, V265, P52