A COMPARISON OF 3 DIFFERENT MAMMALIAN-CELL BIOREACTORS FOR THE PRODUCTION OF MONOCLONAL-ANTIBODIES

被引：4

作者：

PERSSON, B

EMBORG, C

机构：

[1] Center for Process Biotechnology Dept. of Biotechnology Block 223, Technical University of Denmark, Lyngby

来源：

BIOPROCESS ENGINEERING | 1992年 / 8卷 / 3-4期

关键词：

D O I：

10.1007/BF01254232

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three different commercially available stirred tank reactors for mammalian cell culturing were compared for the ability to support hybridoma cell growth and monoclonal antibody production in batch mode operation. Despite quite similar vessel geometries differences were found both in growth and production profiles in the systems. These differences can possibly be related to the different aeration modes used in the bioreactors, and the levels of shear stress created by stirrer and agitator in the tanks.

引用

页码：157 / 163

页数：7

共 45 条

[1] BIOLOGICAL RESPONSES OF HYBRIDOMA CELLS TO DEFINED HYDRODYNAMIC SHEAR-STRESS [J].