CHARACTERIZATION OF A HUMAN CHROMOSOME-4 FLOW-SORTED COSMID LIBRARY

被引：19

作者：

RIESS, O ^{[1
]}

SIEDLACZCK, I ^{[1
]}

KREDTKE, S ^{[1
]}

MELMER, G ^{[1
]}

EPPLEN, JT ^{[1
]}

DEAVEN, LL ^{[1
]}

机构：

[1] LOS ALAMOS NATL LAB, LOS ALAMOS, NM 87545 USA

来源：

CYTOGENETICS AND CELL GENETICS | 1994年 / 65卷 / 04期

关键词：

D O I：

10.1159/000133638

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A cosmid library has been constructed from a hamster x human hybrid cell line and gridded into 270 microtiter plates containing a total of 2 5,920 single colonies. Approximately 84% of the recombinants contain human material, with an average length of 29 kb. This library represents a nearly three-fold coverage of human chromosome 4. We investigated this library for presumptive genes, using a set of oligonucleotides detecting SpI and splice-site consensus sequences. The presence of simple repeat motifs was investigated in the cosmids using the oligonucleotides (GGATTT)3, (GGAT)4, (CAC)5, (GCC)5, (AGC)5, (GATA)4, (GACA)4, and (CA)8 as hybridization probes.

引用

页码：238 / 242

页数：5

共 24 条

[1] CLUES TO THE PATHOGENESIS OF FAMILIAL COLORECTAL-CANCER
AALTONEN, LA
PELTOMAKI, P
LEACH, FS
SISTONEN, P
PYLKKANEN, L
MECKLIN, JP
JARVINEN, H
POWELL, SM
JEN, J
HAMILTON, SR
PETERSEN, GM
KINZLER, KW
VOGELSTEIN, B
DELACHAPELLE, A
[J]. SCIENCE, 1993, 260 (5109) : 812 - 816
[2] SEQUENCE IDENTIFICATION OF 2,375 HUMAN BRAIN GENES
ADAMS, MD
DUBNICK, M
KERLAVAGE, AR
MORENO, R
KELLEY, JM
UTTERBACK, TR
NAGLE, JW
FIELDS, C
VENTER, JC
[J]. NATURE, 1992, 355 (6361) : 632 - 634
[3] ALI S, 1986, HUM GENET, V74, P239
[4] A COSMID CONTIG AND HIGH-RESOLUTION RESTRICTION MAP OF THE 2 MEGABASE REGION CONTAINING THE HUNTINGTONS-DISEASE GENE
BAXENDALE, S
MACDONALD, ME
MOTT, R
FRANCIS, F
LIN, C
KIRBY, SF
JAMES, M
ZEHETNER, G
HUMMERICH, H
VALDES, J
COLLINS, FS
DEAVEN, LJ
GUSELLA, JF
LEHRACH, H
BATES, GP
[J]. NATURE GENETICS, 1993, 4 (02) : 181 - &
[5] MOLECULAR-BASIS OF MYOTONIC-DYSTROPHY - EXPANSION OF A TRINUCLEOTIDE (CTG) REPEAT AT THE 3' END OF A TRANSCRIPT ENCODING A PROTEIN-KINASE FAMILY MEMBER
BROOK, JD
MCCURRACH, ME
HARLEY, HG
BUCKLER, AJ
CHURCH, D
ABURATANI, H
HUNTER, K
STANTON, VP
THIRION, JP
HUDSON, T
SOHN, R
ZEMELMAN, B
SNELL, RG
RUNDLE, SA
CROW, S
DAVIES, J
SHELBOURNE, P
BUXTON, J
JONES, C
JUVONEN, V
JOHNSON, K
HARPER, PS
SHAW, DJ
HOUSMAN, DE
[J]. CELL, 1992, 68 (04) : 799 - 808
[6] CONTINUUM OF OVERLAPPING CLONES SPANNING THE ENTIRE HUMAN CHROMOSOME-21Q
CHUMAKOV, I
RIGAULT, P
GUILLOU, S
OUGEN, P
BILLAUT, A
GUASCONI, G
GERVY, P
LEGALL, I
SOULARUE, P
GRINAS, L
BOUGUELERET, L
BELLANNECHANTELOT, C
LACROIX, B
BARILLOT, E
GESNOUIN, P
POOK, S
VAYSSEIX, G
FRELAT, G
SCHMITZ, A
SAMBUCY, JL
BOSCH, A
ESTIVILL, X
WEISSENBACH, J
VIGNAL, A
RIETHMAN, H
COX, D
PATTERSON, D
GARDINER, K
HATTORI, M
SAKAKI, Y
ICHIKAWA, H
OHKI, M
LEPASLIER, D
HEILIG, R
ANTONARAKIS, S
COHEN, D
[J]. NATURE, 1992, 359 (6394) : 380 - 387
[7] GENOMIC SEQUENCING
CHURCH, GM
GILBERT, W
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07): : 1991 - 1995
[8] CONSTRUCTION OF HUMAN CHROMOSOME-SPECIFIC DNA LIBRARIES FROM FLOW-SORTED CHROMOSOMES
DEAVEN, LL
VANDILLA, MA
BARTHOLDI, MF
CARRANO, AV
CRAM, LS
FUSCOE, JC
GRAY, JW
HILDEBRAND, CE
MOYZIS, RK
PERLMAN, J
[J]. COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1986, 51 : 159 - 167
[9] HIGH-EFFICIENCY VECTORS FOR COSMID MICROCLONING AND GENOMIC ANALYSIS
EVANS, GA
LEWIS, K
ROTHENBERG, BE
[J]. GENE, 1989, 79 (01) : 9 - 20
[10] THE HUMAN Y-CHROMOSOME - OVERLAPPING DNA CLONES SPANNING THE EUCHROMATIC REGION
FOOTE, S
VOLLRATH, D
HILTON, A
PAGE, DC
[J]. SCIENCE, 1992, 258 (5079) : 60 - 66

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