QUANTITATIVE-ANALYSIS OF SYNTHETIC HUMAN CALCITONIN BY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：14

作者：

KOBAYASHI, N ^{[1
]}

KANAI, M ^{[1
]}

SETA, K ^{[1
]}

NAKAMURA, K ^{[1
]}

机构：

[1] FINNIGAN MAT INSTRUMENTS INC,SHIBUYA KU,TOKYO 151,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 672卷 / 01期

关键词：

D O I：

10.1016/0378-4347(95)00205-W

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The quantitative analysis of synthetic human calcitonin (hCT) by micro-liquid chromatography-electrospray ionization mass spectrometry (micro-LC-ESI-MS) is reported. hCT was extracted from plasma by an immobilized antibody column and separated by a micro-LC system. The molecular mass of hCT is 3417, and mit 1140, corresponding to the [M+3H](3+) ion, was observed by ESI-MS. This ion was monitored in the selected-ion monitoring mode; rat calcitonin, which is highly homologous to hCT, was used as an internal standard. The calibration curve for the quantification of hCT was linear in the range 10 ng/ml to 1 mu g/ml of plasma.

引用

页码：17 / 23

页数：7

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