SPATIAL-SCANNING LASER FLUORESCENCE DETECTION FOR CAPILLARY ELECTROPHORESIS

被引：33

作者：

BEALE, SC

SUDMEIER, SJ

机构：

[1] Department of Chemistry, University of Alabama at Birmingham, Alabama, Birmingham

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 18期

关键词：

D O I：

10.1021/ac00114a039

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A laser-induced fluorescence (LIF) detector using epi-illumination and confocal optical detection geometry is described. The LIF detector is designed to scan the entire length of the separation capillary. The capillary is mounted on a precision translational stage which moves the entire capillary through the probe beam. The design of the laser scanner and the results from optimization experiments are presented. The LIF scanner can be used to monitor fluorescence from fluorescein isothiocyanate-labeled proteins focused by capillary isoelectric focusing or to follow the time course of a separation. Dynamically changing the effective separation length is shown to offer a means to decrease analysis time. A method for directly measuring the diffusion coefficient is also presented.

引用

页码：3367 / 3371

页数：5

共 19 条

[1]

ART JJ, 1993, METHOD CELL BIOL, V38, P47

[2] SUBATTOMOLE AMINO-ACID ANALYSIS BY CAPILLARY ZONE ELECTROPHORESIS AND LASER-INDUCED FLUORESCENCE [J].