BIOSEP-SEC-S HIGH-PERFORMANCE SIZE-EXCLUSION CHROMATOGRAPHIC COLUMNS FOR PROTEINS AND PEPTIDES

Spherical silica particles (5-mu-m) of three different pore sizes, 145, 290 and 500 angstrom (Biosep-SEC-S2000, Biosep-SEC-S3000 and Biosep-SEC-S4000), were bonded with a hydrophilic coating and evaluated for the size-exclusion chromatography of proteins and peptides. The results of experiments with synthetic peptides and lysozyme under different mobile phase conditions indicated verv nominal non-specific interaction of proteins with the stationary phases. The recovery of trypsin, protein mass and enzyme activity, determined at mobile phase pH values of 3 and 7, was excellent for all three column types. The new stationary phases showed superior stability towards common protein denaturants and organic modifiers.