EXTRACELLULAR MALTOTETRAOSE-FORMING AMYLASE OF PSEUDOMONAS SP IMD-353

被引：13

作者：

FOGARTY, WM

KELLY, CT

BOURKE, AC

DOYLE, EM

机构：

[1] Department of Industrial Microbiology, University College

来源：

BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 05期

关键词：

D O I：

10.1007/BF01023328

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pseudomonas sp. IMD 353 produced an extracellular consititutive maltotetraose-forming amylase in a medium containing glucose (or fructose), yeatex and mineral salts. Km values an starch, amylopectin and short chain amylose were 4.0, 2.8 and 3.0 mg/ml, respectively. Sulphydryl reducing agents activated the enzyme considerably, as did Co2+ and Mn2+.

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页码：473 / 478

页数：6

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共 17 条

[11] ISOLATION, PURIFICATION, AND CHARACTERIZATION OF A MALTOTETRAOSE-PRODUCING AMYLASE FROM PSEUDOMONAS-STUTZERI [J].

ROBYT, JF ;

ACKERMAN, RJ .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1971, 145 (01) :105-+

[12] PURIFICATION AND PROPERTIES OF AN EXO-ALPHA-AMYLASE FROM PSEUDOMONAS-STUTZERI [J].

SAKANO, Y ;

KASHIWAGI, Y ;

KOBAYASHI, T .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1982, 46 (03) :639-646

[13] PURIFICATION OF ALPHA-MALTOTETRAOSE-FORMING EXO-AMYLASE OF PSEUDOMONAS-STUTZERI - 2-FORMS OF THE AMYLASE AND THEIR ENZYMATIC-PROPERTIES [J].

SAKANO, Y ;

KASHIYAMA, E ;

KOBAYASHI, T .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1983, 47 (08) :1761-1768

[14] STARCH METABOLISM IN PSEUDOMONAS-STUTZERI .1. STUDIES ON MALTOTETRAOSE-FORMING AMYLASE [J].

SCHMIDT, J ;

JOHN, M .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 566 (01) :88-99

[15] STUDIES ON ENZYMATIC PRODUCTION OF OLIGOSACCHARIDES .2. AN AMYLASE PRODUCING MALTOTETRAOSE AND MALTOPENTAOSE FROM BACILLUS-CIRCULANS [J].

TAKASAKI, Y .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1983, 47 (10) :2193-2199

[16] STUDIES ON ENZYMATIC PRODUCTION OF OLIGOSACCHARIDES .1. PRODUCTION OF MALTOHEXAOSE BY ALPHA-AMYLASE FROM BACILLUS CIRCULANS G-6 [J].

TAKASAKI, Y .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1982, 46 (06) :1539-1547

[17] NUCLEOTIDE-SEQUENCE OF THE MALTOTETRAOHYDROLASE GENE FROM PSEUDOMONAS-SACCHAROPHILA [J].

ZHOU, JH ;

BABA, T ;

TAKANO, T ;

KOBAYASHI, S ;

ARAI, Y .

FEBS LETTERS, 1989, 255 (01) :37-41