AN INTRINSICALLY BENT REGION UPSTREAM OF THE TRANSCRIPTION START SITE OF THE RIBOSOMAL-RNA GENES OF ARABIDOPSIS-THALIANA INTERACTS WITH AN HMG-RELATED PROTEIN

被引：12

作者：

KNEIDL, C ^{[1
]}

DINKL, E ^{[1
]}

GRUMMT, F ^{[1
]}

机构：

[1] BIOZENTRUM, INST BIOCHEM, D-97074 WURZBURG, GERMANY

来源：

PLANT MOLECULAR BIOLOGY | 1995年 / 27卷 / 04期

关键词：

ARABIDOPSIS THALIANA; BENT DNA; HIGH MOBILITY GROUP (HMG) PROTEINS; RIBOSOMAL-RNA GENES;

D O I：

10.1007/BF00020224

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intrinsically bent DNA structures are thought to play crucial roles as functionally important modules in promoter/enhancer elements. Here we show the existence of a DNA bending centre within the intergenic region of the tandemly arranged rRNA genes of Arabidopsis thaliana. By use of a circular permutation assay, this bending centre was localized to position -284 to -256 upstream of the transcription start site. A 143 bp fragment containing the bent region was shown to interact with several nuclear proteins. Evidence is presented that one of these proteins is related to the high-mobility-group (HMG) proteins, a group thought to be involved in transcription and replication processes.

引用

页码：705 / 713

页数：9

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