RELATIONSHIP BETWEEN THE ENDOPLASMIC RETICULUM-GOLGI MEMBRANE SYSTEM AND UBIQUINONE BIOSYNTHESIS

被引:30
作者
TECLEBRHAN, H
JAKOBSSONBORIN, A
BRUNK, U
DALLNER, G
机构
[1] LINKOPING UNIV, DEPT PATHOL, S-58185 LINKOPING, SWEDEN
[2] KAROLINSKA INST, NOVUM, CLIN RES CTR, S-14186 HUDDINGE, SWEDEN
来源
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1995年 / 1256卷 / 02期
关键词
ENDOPLASMIC RETICULUM; GOLGI SYSTEM; SUBFRACTIONATION; UBIQUINONE BIOSYNTHESIS;
D O I
10.1016/0005-2760(95)00016-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The involvement of the various segments of the endoplasmic reticulum (ER)-Golgi system in ubiquinone biosynthesis in rat liver was investigated using subcellular fractionation. In addition to preparing rough (R) and smooth microsomes and three different Golgi fractions, a procedure was developed to isolate a smooth vesicle fraction, designated as smooth II (SII) microsomes. The electron micrographs, chemical composition, distribution of marker enzymes, pattern of glycosidases and glycosyltransferases and participation in cholesterol transport suggest that the vesicle components of this latter fraction are intermediary between the endoplasmic reticulum and Golgi system. Both R and smooth I(SI), but not SII microsomes nor Golgi vesicles demonstrate trans-prenyltransferase activity, which synthesizes the side-chain of ubiquinone from geranyl pyrophosphate (GPP). The subsequent enzyme, which transfers solanesyl pyrophosphate (sol-PP) to 4-hydroxybenzoate, is absent from R and SI microsomes, but present in SII microsomes and exhibits high levels of activity in all of the Golgi fractions. Thus, ubiquinone is synthesized sequentially in the ER-Golgi system and thereafter translocated from this compartment to other cellular membranes.
引用
收藏
页码:157 / 165
页数:9
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