STRAINS OF MYCOBACTERIUM-TERRAE COMPLEX WHICH REACT WITH DNA PROBES FOR MYCOBACTERIUM-TUBERCULOSIS COMPLEX

被引:17
作者
FORD, EG [1 ]
SNEAD, SJ [1 ]
TODD, J [1 ]
WARREN, NG [1 ]
机构
[1] COMMONWEALTH VIRGINIA DEPT GEN SERV,DIV CONSOLIDATED LAB SERV,RICHMOND,VA 23219
关键词
D O I
10.1128/JCM.31.10.2805-2806.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Following a recent report that two isolates of Mycobacterium terrae complex had given positive reactions with M. tuberculosis complex DNA probes, a joint study was undertaken to determine the extent of these findings in the clinical culture collection holdings of two state health laboratories. A total of 117 M. terrae complex strains (identified by standard biochemical methods) were subjected to M. tuberculosis complex probe testing with the two then-available kits (from Syngene, Inc., and Gen-Probe, Inc.). In addition to the two original isolates first reported, two further M. terrae complex isolates were found to react with the M. tuberculosis complex probes. Two modifications of the Accuprobe (Gen-Probe, Inc.) test method were evaluated. Extension of the selection time to 8 min was the most convenient modification and rendered the M. terrae complex isolates negative when tested with the Accuprobe M. tuberculosis complex probe. However, the effects of increased selection time on the overall sensitivity of the M. tuberculosis complex probe require further study.
引用
收藏
页码:2805 / 2806
页数:2
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