CIRCULARIZATION OF OLIGONUCLEOTIDES BY DISULFIDE BRIDGE FORMATION

被引：20

作者：

GAO, H ^{[1
]}

YANG, MH ^{[1
]}

PATEL, R ^{[1
]}

COOK, AF ^{[1
]}

机构：

[1] PHARMAGEN INC, ALLENDALE, NJ 07401 USA

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 11期

关键词：

D O I：

10.1093/nar/23.11.2025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An effective, convenient method for the circularization of oligonucleotides has been developed, This procedure involved preparation of an oligonucleotide with backbone-linked 5'- and 3'-terminal hexamethylenethiol groups, followed by oxidation of the thiol groups with air or oxygen to produce the corresponding circular sequence bridged via a bis(hexamethylene)disulfide moiety, The method has been applied to the circularization of oligodeoxynucleotide sequences of varying lengths (5, 10, 15, 20, 30 and 40 bases), and the circularization process was highly efficient as shown by HPLC or gel electrophoresis of the crude reaction mixtures, Competing reactions such as dimerization were not significant except for the longer sequences (30 and 40 bases), The circularization of an eight base RNA sequence was also accomplished, as well as hexa-ethylene glycol bridged poly-T sequences capable of tripler formation.

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页码：2025 / 2029

页数：5

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