A HIGH-RESOLUTION MAP OF THE VICINITY OF THE R1 LOCUS ON CHROMOSOME-V OF POTATO BASED ON RFLP AND AFLP MARKERS

被引：209

作者：

MEKSEM, K

LEISTER, D

PELEMAN, J

ZABEAU, M

SALAMINI, F

GEBHARDT, C

机构：

[1] MAX PLANCK INST ZUCHTUNGSFORSCH,D-50829 COLOGNE,GERMANY

[2] KEYGENE NV,6700 AE WAGENINGEN,NETHERLANDS

来源：

MOLECULAR & GENERAL GENETICS | 1995年 / 249卷 / 01期

关键词：

POTATO; PHYTOPHTHORA INFESTANS; RESISTANCE; MAP-BASED CLONING; AFLP MARKERS;

D O I：

10.1007/BF00290238

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The R1 allele confers on potato a race-specific resistance to Phytophthora infestans. The corresponding genetic locus maps on chromosome V in a region in which several other resistance genes are also located. As part of a strategy for cloning R1, a high-resolution genetic map was constructed for the segment of chromosome V that is bordered by the RFLP loci GP21 and GP179 and includes the R1 locus. Bulked segregant analysis and markers based on amplified fragment length polymorphisms (AFLP markers) were used to select molecular markers closely linked to R1. Twentynine of approximately 3200 informative AFLP loci displayed linkage to the R1 locus. Based on the genotypic analysis of 461 gametes, eight loci mapped within the GP21-GP179 interval. Two of those could not be seperated from R1 by recombination. For genotyping large numbers of plants with respect to the flanking markers GP21 and GP179 PCR based assays were also developed which allowed marker-assisted selection of plants with genotypes Rr and rr and of recombinant plants.

引用

页码：74 / 81

页数：8

共 31 条

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