POTENTIOMETRIC DETERMINATION OF INORGANIC-PHOSPHATE USING A SALICYLATE-SENSITIVE MEMBRANE-ELECTRODE AND AN ALKALINE-PHOSPHATASE ENZYME

被引：19

作者：

KATSU, T

KAYAMOTO, T

机构：

[1] Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 265卷 / 01期

关键词：

ENZYMATIC METHODS; POTENTIOMETRY; BLOOD; PHOSPHATE; SERUM;

D O I：

10.1016/0003-2670(92)85147-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for determining inorganic phosphate using a salicylate-sensitive membrane electrode is described, based on the detection of salicylate formed by enzymatic hydrolysis of o-carboxyphenyl phosphate by alkaline phosphatase in the presence of phosphate. The phosphate inhibited competitively the formation of salicylate to an extent inversely proportional to the concentration of added phosphate, detected down to 0.05 mM. The method was successfully applied to the determination of phosphate in blood serum.

引用

页码：1 / 4

页数：4

共 8 条

[1]

CHANG R, 1981, PHYSICAL CHEM APPLIC, P390

[2]

Fernley H.N., 1971, ENZYMES, V4, P417

[3] A FINE-STRUCTURE GENETIC AND CHEMICAL STUDY OF THE ENZYME ALKALINE PHOSPHATASE OF E-COLI .1. PURIFICATION AND CHARACTERIZATION OF ALKALINE PHOSPHATASE [J].