TISSUE-SPECIFIC GENE ACTIVATION BY MYOD - DETERMINATION OF SPECIFICITY BY CIS-ACTING REPRESSION ELEMENTS

被引:83
作者
WEINTRAUB, H
GENETTA, T
KADESCH, T
机构
[1] UNIV PENN,SCH MED,HOWARD HUGHES MED INST,PHILADELPHIA,PA 19104
[2] UNIV PENN,SCH MED,DEPT GENET,PHILADELPHIA,PA 19104
关键词
MYOD; TRANSCRIPTIONAL REPRESSION; E BOX; IGH ENHANCER;
D O I
10.1101/gad.8.18.2203
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
MyoD is a muscle-specific transcriptional activator; E12 is a B-cell activator. An IgH enhancer is activated almost 100-fold by E12 but not at all by MyoD; an MCK enhancer is activated almost 1000-fold by MyoD and not at all by E12. MyoD and E12 are both basic helix-loop-helix proteins that bind to similar E-box sequences (CANNTG); the IgH enhancer contains the same E boxes as the MCK enhancer, yet each retains exclusive specificity for either E12 or MyoD, respectively. We show that the IgH enhancer contains a cis-acting negative element that is directed at MyoD, but not at E12. This repression requires the mu E5 E box within the IgH enhancer; however, the specificity for repression, as opposed to activation, is associated with 2 bp flanking each side of the mu E5 E box. The target for repression of MyoD in the IgH enhancer is the bHLH region of MyoD. Our results suggest that MyoD only activates myogenic genes because nonmuscle enhancers that contain E boxes also contain negative elements that prevent MyoD activity.
引用
收藏
页码:2203 / 2211
页数:9
相关论文
共 30 条