DEPTH RESOLUTION OF THE FLUORESCENT CONFOCAL SCANNING OPTICAL MICROSCOPE

被引：10

作者：

KIMURA, S

MUNAKATA, C

机构：

[1] Hitachi Ltd., Central Research Laboratory, Kokubunii, Tokyo

来源：

APPLIED OPTICS | 1990年 / 29卷 / 04期

关键词：

D O I：

10.1364/AO.29.000489

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The fluorescent confocal scanning optical microscope is capable of 3-D observation, for which depth discrimination is vital. To characterize the depth discrimination capability of this microscope, intensity variations depending on fluorescence wavelength are simulated for fluorescent films being moved in the depth direction. Also, the resolution between two films is calculated for a range of fluorescence wavelengths. Results of experiments using photoresist films are compared to simulations. As a practical application, through-holes opened in a thick photoresist film are observed by slice images in the depth direction. © 1990 Optical Society of America.

引用

页码：489 / 494

页数：6

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