3-AMINOPICOLINIC AID AS A MATRIX FOR LASER-DESORPTION MASS-SPECTROMETRY OF BIOPOLYMERS

被引：25

作者：

TARANENKO, NI ^{[1
]}

TANG, K ^{[1
]}

ALLMAN, SL ^{[1
]}

CHANG, LY ^{[1
]}

CHEN, CH ^{[1
]}

机构：

[1] OAK RIDGE NATL LAB,OAK RIDGE,TN 37831

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1994年 / 8卷 / 12期

关键词：

D O I：

10.1002/rcm.1290081219

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

3-Aminopicolinic acid (3-APA) was tested and feued to be a useful matrix for matrix-assisted laser desorption/ionization of DNA and protein. Single-stranded DNA segments of 150-mer and double-stranded DNA of 246 base pairs were successfully detected by using 3-APA as an ultraviolet-absorbing matrix in a linear time-of-flight mass spectrometer. In the case of the double-stranded DNA, only parent ions corresponding to single-stranded DNA were observed. The comparison with 3-hydroxypicolinic acid and picolinic acid matrices is discussed.

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页码：1001 / 1006

页数：6

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