DETERMINATION OF 25-HYDROXYVITAMIN-D-3 IN HUMAN PLASMA USING A NONRADIOACTIVE TETRANORVITAMIN-D ANALOG AS AN INTERNAL STANDARD

被引：11

作者：

SHIMIZU, M ^{[1
]}

IWASAKI, Y ^{[1
]}

ISHIDA, H ^{[1
]}

YAMADA, S ^{[1
]}

机构：

[1] TOKYO MED & DENT UNIV,INST MED & DENT ENGN,DIV MOLEC BIOL,CHIYODA KU,TOKYO 101,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 672卷 / 01期

关键词：

D O I：

10.1016/0378-4347(95)00187-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A convenient non-radioactive method for assaying plasma 25-hydroxyvitamin D-3, is described. The method uses 22-hydroxytetranorvitamin D-3 as an internal standard and includes two-step liquid- and solid-phase extractions and quantification by normal-phase HPLC. The intra- and inter-assay coefficients of variation were 2.2% and 2.4%, respectively, and the analytical recovery of 25-hydroxyvitamin D-3 added to plasma was quantitative. Assay linearity was obtained in the range 0.5-4.0 mi of plasma. When compared with the method employing a radioactive 25-hydroxyvitamin D-3 tracer, the correlation coefficient was 0.990 (slope 0.999 and intercept 1.19 ng/ml).

引用

页码：63 / 71

页数：9

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