INHIBITION OF CDP-DG - INOSITOL TRANSFERASE BY INOSTAMYCIN

被引：23

作者：

IMOTO, M ^{[1
]}

TANIGUCHI, Y ^{[1
]}

UMEZAWA, K ^{[1
]}

机构：

[1] KEIO UNIV,FAC SCI & TECHNOL,APPL CHEM,3-14-1 HIYOSHI,KOHOKU KU,YOKOHAMA,KANAGAWA 223,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1992年 / 112卷 / 02期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123894

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inostamycin, a novel microbial secondary metabolite, inhibited [H-3]inositol and P-32(i) incorporation into phosphatidylinositol (PtdIns) induced by epidermal growth factor (EGF) in cultured A431 cells, the IC50 being 0.5-mu-g/ml, without inhibiting macromolecular synthesis. The drug inhibited cellular inositol phosphate formation only when it was added at the same time as labeled inositol. It was found to inhibit in vitro CDP-DG:inositol transferase activity of the A431 cell membrane, the IC50 being about 0.02-mu-g/ml. It did not inhibit tyrosine kinase, PtdIns phospholipase C, or PtdIns kinase. Therefore, inhibition of PtdIns turnover by inostamycin must be due to the inhibition of CDP-DG: inositol transferase. Thus, inostamycin is a novel inhibitor of CDP-DG:inositol transferase.

引用

页码：299 / 302

页数：4

共 15 条

[1] INOSITOL TRISPHOSPHATE FORMATION AND CALCIUM MOBILIZATION IN SWISS 3T3 CELLS IN RESPONSE TO PLATELET-DERIVED GROWTH-FACTOR
BERRIDGE, MJ
HESLOP, JP
IRVINE, RF
BROWN, KD
[J]. BIOCHEMICAL JOURNAL, 1984, 222 (01) : 195 - 201
[2] INOSITOL TRISPHOSPHATE, A NOVEL 2ND MESSENGER IN CELLULAR SIGNAL TRANSDUCTION
BERRIDGE, MJ
IRVINE, RF
[J]. NATURE, 1984, 312 (5992) : 315 - 321
[3] ON SPECIFICITY OF CYTIDINE DIPHOSPHATE DIGLYCERIDES IN MONOPHOSPHOINOSITIDE BIOSYNTHESIS BY RAT BRAIN PREPARATIONS
BISHOP, HH
STRICKLAND, KP
[J]. CANADIAN JOURNAL OF BIOCHEMISTRY, 1970, 48 (03): : 269 - +
[4] RAS-TRANSFORMED CELLS - ALTERED LEVELS OF PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE AND CATABOLITES
FLEISCHMAN, LF
CHAHWALA, SB
CANTLEY, L
[J]. SCIENCE, 1986, 231 (4736) : 407 - 410
[5] HASEGAWASASAKI H, 1988, J BIOL CHEM, V263, P12970
[6] ISOLATION AND STRUCTURE DETERMINATION OF INOSTAMYCIN, A NOVEL INHIBITOR OF PHOSPHATIDYLINOSITOL TURNOVER
IMOTO, M
UMEZAWA, K
TAKAHASHI, Y
NAGANAWA, H
IITAKA, Y
NAKAMURA, H
KOIZUMI, Y
SASAKI, Y
HAMADA, M
SAWA, T
TAKEUCHI, T
[J]. JOURNAL OF NATURAL PRODUCTS, 1990, 53 (04): : 825 - 829
[7] INHIBITION OF CELLULAR PHOSPHATIDYLINOSITOL TURNOVER BY PSI-TECTORIGENIN
IMOTO, M
YAMASHITA, T
SAWA, T
KURASAWA, S
NAGANAWA, H
TAKEUCHI, T
BAOQUAN, Z
UMEZAWA, K
[J]. FEBS LETTERS, 1988, 230 (1-2) : 43 - 46
[8] INHIBITION OF EGF-INDUCED PHOSPHOLIPASE-C ACTIVATION IN A431 CELLS BY ERBSTATIN, A TYROSINE KINASE INHIBITOR
IMOTO, M
SHIMURA, N
UI, H
UMEZAWA, K
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 173 (01) : 208 - 211
[9] INHIBITION OF EPIDERMAL GROWTH FACTOR-INDUCED ACTIVATION OF PHOSPHOLIPASE-C BY PSI-TECTORIGENIN
IMOTO, M
SHIMURA, N
UMEZAWA, K
[J]. JOURNAL OF ANTIBIOTICS, 1991, 44 (08) : 915 - 917
[10] JACKOWSKI S, 1986, J BIOL CHEM, V261, P4978

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