ISOLATION AND PRELIMINARY CHARACTERIZATION OF PICHIA-PINUS MUTANTS INSENSITIVE TO GLUCOSE REPRESSION

被引：10

作者：

ALAMAE, T

SIMISKER, J

机构：

[1] Institute of Molecular and Cell Biology, University of Tartu, Tartu, EE2400

来源：

YEAST | 1994年 / 10卷 / 11期

关键词：

PICHIA PINUS; GLUCOSE CATABOLITE REPRESSION; GLUCOSE TRANSPORT; ISOLATION OF MUTANTS;

D O I：

10.1002/yea.320101109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for the isolation of glucose repression-insensitive mutants in the methylotrophic yeast Pichia pinus was developed. The method is based on screening of small suspension samples derived from 2-deoxyglucose-resistant colonies for alcohol oxidase activity. Alcohol oxidase activity was evaluated by determination of formaldehyde excreted by cells. Mutants with glucose non-repressible alcohol oxidase and catalase synthesis were obtained. All mutants grew poorly on D-xylose compared to the wild type, whereas growth on L-arabinose was similar to the wild type. Changes in the glucose transport system were suggested to be responsible for altered growth characteristics and defective glucose repression.

引用

页码：1459 / 1466

页数：8

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