CRYSTALLIZATION OF A HUMICOLA-LANUGINOSA LIPASE-INHIBITOR COMPLEX WITH THE USE OF POLYETHYLENE-GLYCOL MONOMETHYL ETHER

The fungal Humicola lanuginosa lipase complexed with the inhibitor n-dodecylphosphonate ethyl ester was crystallized in space group P2(1)2(1)2(1) with pseudotetragonal unit-cell parameters of a = 131.7 (2), b = 131.3 (1), c = 75.4 (1) angstrom. 92% of X-ray diffraction data to 2.8 angstrom resolution were collected with a final R(merge) = 8.5%. The crystals were grown using a new kind of precipitant - polyethylene glycol monomethyl ether (Peg-mme) of molecular weight 5000.