A PRELIMINARY-STUDY OF THE EFFECT OF GROWTH-HORMONE ON MOUSE PREIMPLANTATION EMBRYO DEVELOPMENT IN-VITRO

被引：15

作者：

DRAKAKIS, P ^{[1
]}

LOUTRADIS, D ^{[1
]}

MILINGOS, S ^{[1
]}

MICHALAS, S ^{[1
]}

KALLIANIDIS, K ^{[1
]}

BLETSA, R ^{[1
]}

ARAVANTINOS, D ^{[1
]}

KIESSLING, AA ^{[1
]}

机构：

[1] HARVARD UNIV,FAULKNER HOSP,SCH MED,FAULKNER CTR REPROD MED,BOSTON,MA 02115

来源：

GYNECOLOGIC AND OBSTETRIC INVESTIGATION | 1995年 / 40卷 / 04期

关键词：

GROWTH HORMONE; MOUSE PREIMPLANTATION EMBRYOS; IN VITRO BLASTOCYST DEVELOPMENT; HATCHING;

D O I：

10.1159/000292341

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The role of growth hormone (GH) in follicular development, ovulation and embryo development is currently under reconsideration. In this study, we have tried to investigate the effect of GH on preimplantation development. of mouse embryos in vitro. Zygotes and two-cell mouse embryos were cultured without (control) or with GH. For zygotes, the addition of 0.2 mu g/ml of GH resulted in 77 +/- 1% of blastocysts formed and 66 +/- 3% rate of hatching (control 64 +/- 4 and 31 +/- 3%, p < 0.05 and p < 0.01, respectively). For two-cell embryos, the addition of 0.2 mu g/ml of GH resulted in 87 +/- 2% of blastocysts formed and 60 +/- 4% hatching rate (control 76 +/- 4 and 47 +/- 5%, p < 0.05 for both). This positive effect of GH addition implies that the latter can support mouse preimplantation development in vitro and it suggests, along with its local action on the ovary and its possible effects, via the insulin-like growth factor system, on the tubal and uterine epithelium, a continuous role of this hormone in reproductive physiology from follicular maturation to embryonic development and, possibly, implantation.

引用

页码：222 / 226

页数：5

共 34 条

[1] BENEFICIAL EFFECT OF EDTA ON DEVELOPMENT OF MOUSE ONE-CELL EMBRYOS IN CHEMICALLY DEFINED MEDIUM [J].

ABRAMCZUK, J ;

SOLTER, D ;

KOPROWSKI, H .

DEVELOPMENTAL BIOLOGY, 1977, 61 (02) :378-383

[2] INSULIN-LIKE GROWTH-FACTORS - THE OVARIAN CONNECTION [J].

ADASHI, EY ;

RESNICK, CE ;

HURWITZ, A ;

RICCIARELLI, E ;

HERNANDEZ, ER ;

ROBERTS, CT ;

LEROITH, D ;

ROSENFELD, R .

HUMAN REPRODUCTION, 1991, 6 (09) :1213-1219

[3] IMPROVED QUALITY OF HUMAN-EMBRYOS WHEN CO-CULTURED WITH HUMAN AMPULLARY CELLS [J].

BONGSO, A ;

NG, SC ;

SATHANANTHAN, H ;

NG, PL ;

RAUFF, M ;

RATNAM, S .

HUMAN REPRODUCTION, 1989, 4 (06) :706-713

[4]

BONGSO A, 1991, FERTIL STERIL, V56, P179

[5]

CHATOT CL, 1989, J REPROD FERTIL, V86, P679, DOI 10.1530/jrf.0.0860679

[6]

CHRISTMAN GM, 1992, FERTIL STERIL, V57, P12

[7]

DAVIDSON A, 1988, FERTIL STERIL, V49, P516

[8] INSULIN-ANTIBODIES RETARD AND INSULIN ACCELERATES GROWTH AND DIFFERENTIATION IN EARLY EMBRYOS [J].

DEPABLO, F ;

GIRBAU, M ;

GOMEZ, JA ;

HERNANDEZ, E ;

ROTH, J .

DIABETES, 1985, 34 (10) :1063-1067

[9] TEMPORAL EFFECTS OF TAURINE ON MOUSE PREIMPLANTATION DEVELOPMENT INVITRO [J].

DUMOULIN, JCM ;

EVERS, JLH ;

BAKKER, JA ;

BRAS, M ;

PIETERS, MHEC ;

GERAEDTS, JPM .

HUMAN REPRODUCTION, 1992, 7 (03) :403-407

[10]

EDWARDS RG, 1980, BRIT J OBSTET GYNAEC, V87, P737, DOI 10.1111/j.1471-0528.1980.tb04610.x

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