FIBROBLAST GROWTH-FACTOR ENHANCES THE TRANSCRIPTION AND STABILITY OF HUMAN CHORIONIC-GONADOTROPIN BETA-SUBUNIT MESSENGER-RIBONUCLEIC-ACID IN JAR CHORIOCARCINOMA CELLS
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作者:
OBERBAUER, AM
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机构:LOMA LINDA UNIV, DIV PERINATAL BIOL, LOMA LINDA, CA 92350 USA
OBERBAUER, AM
STRONG, DD
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机构:LOMA LINDA UNIV, DIV PERINATAL BIOL, LOMA LINDA, CA 92350 USA
STRONG, DD
LINKHART, TA
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机构:LOMA LINDA UNIV, DIV PERINATAL BIOL, LOMA LINDA, CA 92350 USA
LINKHART, TA
MOHAN, S
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机构:LOMA LINDA UNIV, DIV PERINATAL BIOL, LOMA LINDA, CA 92350 USA
MOHAN, S
LONGO, LD
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机构:LOMA LINDA UNIV, DIV PERINATAL BIOL, LOMA LINDA, CA 92350 USA
LONGO, LD
机构:
[1] LOMA LINDA UNIV, DIV PERINATAL BIOL, LOMA LINDA, CA 92350 USA
[2] LOMA LINDA UNIV, DEPT PHYSIOL, LOMA LINDA, CA 92350 USA
[3] LOMA LINDA UNIV, DEPT BIOCHEM, LOMA LINDA, CA 92350 USA
[4] LOMA LINDA UNIV, DEPT PEDIAT, LOMA LINDA, CA 92350 USA
[5] LOMA LINDA UNIV, DEPT MED, LOMA LINDA, CA 92350 USA
[6] JERRY L PETTIS VET HOSP, MINERAL METAB LAB, LOMA LINDA, CA 92354 USA
In previous studies, we found that basic fibroblast growth factor (bFGF) significantly stimulated the secretion of hCGbeta in the Jar choriocarcinoma cell line. In the present study, the effect of bFGF on the steady state hCGbeta mRNA level in this cell line was determined. Application of Northern analyses with total RNA isolated from bFGF-stimulated Jar cells revealed that, in a time-dependent manner, the steady state hCGbeta mRNA level increased progressively, reaching 4-fold of the control value within 4 h after exposure to bFGF. The observed accumulation was due in part to increased transcription (2.4-fold relative to that in control cultures), as determined by nuclear transcription studies. In addition, bFGF increased the stability of the hCGbeta message; the message half-life was increased from approximately 3 h (in control cultures) to greater than 6 h (in bFGF-treated cultures). These data demonstrate that bFGF stimulates hCGbeta mRNA accumulation in a complex manner regulated through both transcriptional and posttranscriptional mechanisms.