DETERMINATION OF SULFITE BY USE OF A FIBEROPTIC BIOSENSOR BASED ON A CHEMILUMINESCENT REACTION

被引：24

作者：

HLAVAY, J ^{[1
]}

GUILBAULT, GG ^{[1
]}

机构：

[1] UNIV NEW ORLEANS,DEPT CHEM,NEW ORLEANS,LA 70148

来源：

ANALYTICA CHIMICA ACTA | 1994年 / 299卷 / 01期

基金：

匈牙利科学研究基金会;

关键词：

BIOSENSORS; CHEMILUMINESCENCE; FIBEROPTIC BIOSENSOR; SULFITE;

D O I：

10.1016/0003-2670(94)00331-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fiber-optic biosensor based on the immobilization of sulphite oxidase and peroxidase on different membranes, and subsequent detection of luminol chemiluminescence has been developed. The response was linear in the concentration ranges of 1 to 100 mu M and 3.2 to 320 mu M, with a detection limit (signal-to-noise ratio=3) of 0.5 mu M sulphite. A Nucleopore polycarbonate membrane proved to be superior to the chemically preactivated ones. The effect of pH, buffer, and immobilization conditions, as well as the elimination of ascorbic acid interference are also discussed. The feasibility of the biosensor developed was demonstrated by assaying the sulphite content of different food samples.

引用

页码：91 / 96

页数：6

共 17 条

[1] PEROXIDE OPTRODE BASED ON MICELLAR-MEDIATED CHEMI-LUMINESCENCE REACTION OF LUMINOL [J].