DETERMINATION OF AFLATOXINS BY REVERSED-PHASE HIGHPERFORMANCE LIQUID-CHROMATOGRAPHY WITH POSTCOLUMN IN-LINE PHOTOCHEMICAL DERIVATIZATION AND FLUORESCENCE DETECTION

被引：78

作者：

JOSHUA, H ^{[1
]}

机构：

[1] MERCK & CO INC,RES LABS,RAHWAY,NJ 07065

来源：

JOURNAL OF CHROMATOGRAPHY A | 1993年 / 654卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)83367-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Post-column in-line photochemical derivatization permits fluorescence detection of all four common aflatoxins B-1, B-2, G(1) and G(2). Chromatographic evidence indicates that the photolysis causes the hydration of the non-fluorescent B-1 and G(1) components to the B-2a and G(2a c) omponents respectively. Analysis of naturally contaminated corn samples show no interfering peaks and permits the determination of 1 and 0.25 ppb (w/w) of B-1 and B-2, respectively.

引用

页码：247 / 254

页数：8

共 24 条

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