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MUTATION DETECTION BY DENATURING GRADIENT ELECTROPHORESIS (DGGE)

被引：156
作者：
FODDE, R
LOSEKOOT, M
机构：
[1] MGC-Department of Human Genetics, Sylvius Laboratorium, Leiden
来源：
HUMAN MUTATION | 1994年 / 3卷 / 02期
关键词：
DGGE; MUTATION; PCR; SSCP; HOT;
D O I：
10.1002/humu.1380030202
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The molecular analysis of genetic diseases relies on several technical approaches which allow genetic and physical mapping, characterization of the gene structure, expression studies, and identification of disease-causing mutations. Denaturing gradient gel electrophoresis (DGGE) allows the rapid screening for single base changes in enzymatically amplified DNA. The technique is based on the migration of double stranded DNA molecules through polyacrylamide gels containing linearly increasing concentrations of a denaturing agent. In this review DGGE and the several modifications of the original protocol are presented. Moreover, its applications in human molecular genetics are summarized together with a preliminary comparison with other mutation detection technologies such as chemical cleavage, RNase protection, and single strand conformation polymorphism. (C) 1994 Wiley-Liss, Inc.
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页码：83 / 94
页数：12
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