SCANNING FORCE MICROSCOPY AND CRYOELECTRON MICROSCOPY OF TOBACCO MOSAIC-VIRUS AS A TEST SPECIMEN

被引：44

作者：

ZENHAUSERN, F ^{[1
]}

ADRIAN, M ^{[1
]}

EMCH, R ^{[1
]}

TABORELLI, M ^{[1
]}

JOBIN, M ^{[1
]}

DESCOUTS, P ^{[1
]}

机构：

[1] UNIV LAUSANNE,ANAL ULTRASTRUCT LAB,CH-1015 LAUSANNE,SWITZERLAND

来源：

ULTRAMICROSCOPY | 1992年 / 42卷

关键词：

D O I：

10.1016/0304-3991(92)90419-K

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

In this study, tobacco mosaic virus (TMV) provides a resolution criterion for specimen preparation methods as well as for imaging parameters of the scanning force microscope (SFM). We present scanning force microscopic images of the virus embedded in 0.5% buffered phosphotungstic acid solution adsorbed on a freshly cleaved mica surface, and imaged under atmospheric conditions. Individual TMV particles were clearly identified with a characteristic shape of long rods of about 300 nm long and 60-70 nm in apparent width due to the geometric parameters of the tip. The structure of the virus was compared with cryo-electron microscopic data of vitrified suspensions observed to a resolution of 1.15 nm. Uncoated TMV particles were also deposited on evaporated titanium thin films and imaged by SFM.

引用

页码：1168 / 1172

页数：5