IMMOBILIZED PROTEINS IN BUFFER IMAGED AT MOLECULAR RESOLUTION BY ATOMIC FORCE MICROSCOPY

被引：164

作者：

WEISENHORN, AL ^{[1
]}

DRAKE, B ^{[1
]}

PRATER, CB ^{[1
]}

GOULD, SAC ^{[1
]}

HANSMA, PK ^{[1
]}

OHNESORGE, F ^{[1
]}

EGGER, M ^{[1
]}

HEYN, SP ^{[1
]}

GAUB, HE ^{[1
]}

机构：

[1] TECH UNIV MUNICH,DEPT PHYS,W-8046 GARCHING,GERMANY

来源：

BIOPHYSICAL JOURNAL | 1990年 / 58卷 / 05期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0006-3495(90)82465-6

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Samples of supported planar lipid-protein membranes and actin filaments on mica were imaged by atomic force microscopy (AFM). The samples were fully submerged in buffer at room temperature during imaging. Individual proteins bound to the reconstituted membrane were distinguishable; some structural details could be resolved. Also, surface-induced, self-assembling of actin filaments on mica could be observed. Monomeric subunits were imaged on individual actin filaments. The filaments could be manipulated on or removed from the surface by the tip of the AFM. The process of the decoupling of the filamentous network from the surface upon changing the ionic conditions was imaged in real time. © 1990, The Biophysical Society. All rights reserved.

引用

页码：1251 / 1258

页数：8

共 27 条

[1] ATOMIC RESOLUTION WITH THE ATOMIC FORCE MICROSCOPE ON CONDUCTORS AND NONCONDUCTORS
ALBRECHT, TR
QUATE, CF
[J]. JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS, 1988, 6 (02): : 271 - 274
[2] AN ATOMIC-RESOLUTION ATOMIC-FORCE MICROSCOPE IMPLEMENTED USING AN OPTICAL-LEVER
ALEXANDER, S
HELLEMANS, L
MARTI, O
SCHNEIR, J
ELINGS, V
HANSMA, PK
LONGMIRE, M
GURLEY, J
[J]. JOURNAL OF APPLIED PHYSICS, 1989, 65 (01) : 164 - 167
[3] AMER NM, 1988, B AM PHYS SOC, V33, P319
[4] MONOCLONAL-ANTIBODIES TO A NITROXIDE LIPID HAPTEN
BALAKRISHNAN, K
HSU, FJ
HAFEMAN, DG
MCCONNELL, HM
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 721 (01) : 30 - 38
[5] ATOMIC FORCE MICROSCOPE
BINNIG, G
QUATE, CF
GERBER, C
[J]. PHYSICAL REVIEW LETTERS, 1986, 56 (09) : 930 - 933
[6] ATOMIC RESOLUTION WITH ATOMIC FORCE MICROSCOPE
BINNIG, G
GERBER, C
STOLL, E
ALBRECHT, TR
QUATE, CF
[J]. EUROPHYSICS LETTERS, 1987, 3 (12): : 1281 - 1286
[7] NEUTRON-DIFFRACTION STUDIES ON SELECTIVELY DEUTERATED PHOSPHOLIPID BILAYERS
BULDT, G
GALLY, HU
SEELIG, A
SEELIG, J
[J]. NATURE, 1978, 271 (5641) : 182 - 184
[8] 3-DIMENSIONAL STRUCTURE OF IMMUNOGLOBULINS
DAVIES, DR
PADLAN, EA
SEGAL, DM
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1975, 44 : 639 - 667
[9] IMAGING CRYSTALS, POLYMERS, AND PROCESSES IN WATER WITH THE ATOMIC FORCE MICROSCOPE
DRAKE, B
PRATER, CB
WEISENHORN, AL
GOULD, SAC
ALBRECHT, TR
QUATE, CF
CANNELL, DS
HANSMA, HG
HANSMA, PK
[J]. SCIENCE, 1989, 243 (4898) : 1586 - 1589
[10] 2-DIMENSIONAL RECOGNITION PATTERN OF LIPID-ANCHORED FAB' FRAGMENTS
EGGER, M
HEYN, SP
GAUB, HE
[J]. BIOPHYSICAL JOURNAL, 1990, 57 (03) : 669 - 673

← 1 2 3 →