CAPILLARY ELECTROPHORESIS OF DNA FRAGMENTS IN ENTANGLED POLYMER-SOLUTIONS - A STUDY OF SEPARATION VARIABLES

被引：39

作者：

DOLNIK, V ^{[1
]}

NOVOTNY, M ^{[1
]}

机构：

[1] INDIANA UNIV,DEPT CHEM,BLOOMINGTON,IN 47405

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1992年 / 4卷 / 06期

关键词：

CAPILLARY ELECTROPHORESIS; ENTANGLED POLYMER SOLUTIONS; PBR322 DNA HAE-III DIGEST; GALACTOMANNAN; SYNERGEL(TM);

D O I：

10.1002/mcs.1220040609

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary electrophoresis (CE) in entangled polymer solutions was studied using pBR322 DNA Hae III digest as a model sample. The influences of polymer nature, monomer chain length, and polymer concentration were investigated. The use of a novel polymer, galactomannan, as the entangled matrix for the separation of DNA fragments has been demonstrated.

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页码：515 / 519

页数：5

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