FUNCTIONAL-CHARACTERIZATION OF THE STAPHYLOCOCCUS-CARNOSUS SECA PROTEIN IN ESCHERICHIA-COLI AND BACILLUS-SUBTILIS SECA MUTANT STRAINS

被引：16

作者：

KLEIN, M ^{[1
]}

MEENS, J ^{[1
]}

FREUDL, R ^{[1
]}

机构：

[1] FORSCHUNGSZENTRUM JULICH, FORSCHUNGSZENTRUM, INST BIOTECHNOL 1, D-52425 JULICH, GERMANY

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 131卷 / 03期

关键词：

STAPHYLOCOCCUS CARNOSUS; PROTEIN TRANSLOCATION; SECA PROTEIN; PREPROTEIN TRANSLOCASE;

D O I：

10.1016/0378-1097(95)00267-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Staphylococcus carnosus secA gene was cloned using the Bacillus subtilis secA gene as a probe. The S. carnosus secA encodes a polypeptide of 844 amino acid residues which is homologous to other known SecA proteins. The S. carnosus SecA functionally complemented the growth and secretion defects of a temperature-sensitive B. subtilis secA mutant at the non-permissive temperature. In contrast, the growth defect of an Escherichia coli secA mutant could not be complemented by the S. carnosus SecA protein. Our results suggest that the interactions of SecA with precursor proteins and/or other components of bacterial preprotein translocase are optimized within each organism.

引用

页码：271 / 277

页数：7

共 25 条

[1] TIGHTLY REGULATED TAC PROMOTER VECTORS USEFUL FOR THE EXPRESSION OF UNFUSED AND FUSED PROTEINS IN ESCHERICHIA-COLI [J].

AMANN, E ;

OCHS, B ;

ABEL, KJ .

GENE, 1988, 69 (02) :301-315

[2] THE BACILLUS-SUBTILIS SIGMA(D)-DEPENDENT OPERON ENCODING THE FLAGELLAR PROTEINS FLID, FLIS, AND FLIT [J].

CHEN, L ;

HELMANN, JD .

JOURNAL OF BACTERIOLOGY, 1994, 176 (11) :3093-3101

[3] BACTERIAL PROTEIN TRANSLOCATION - KINETIC AND THERMODYNAMIC ROLE OF ATP AND THE PROTONMOTIVE FORCE [J].

DRIESSEN, AJM .

TRENDS IN BIOCHEMICAL SCIENCES, 1992, 17 (06) :219-223

[4] SECA PROMOTES PREPROTEIN TRANSLOCATION BY UNDERGOING ATP-DRIVEN CYCLES OF MEMBRANE INSERTION AND DEINSERTION [J].

ECONOMOU, A ;

WICKNER, W .

CELL, 1994, 78 (05) :835-843

[5] ISOLATION AND CHARACTERIZATION OF THE SECE HOMOLOG GENE OF BACILLUS-SUBTILIS [J].

JEONG, SM ;

YOSHIKAWA, H ;

TAKAHASHI, H .

MOLECULAR MICROBIOLOGY, 1993, 10 (01) :133-142

[6]

KLEIN M, 1994, FEMS MICROBIOL LETT, V124, P393

[7]

KLOSE M, 1993, J BIOL CHEM, V268, P4504

[8] THE STAPHYLOCOCCUS-CARNOSUS SECE GENE - CLONING, NUCLEOTIDE-SEQUENCE, AND FUNCTIONAL-CHARACTERIZATION IN ESCHERICHIA-COLI SECE MUTANT STRAINS [J].

MEENS, J ;

KLOSE, M ;

FREUDL, R .

FEMS MICROBIOLOGY LETTERS, 1994, 117 (01) :113-119

[9] AN OUTER-MEMBRANE PROTEIN (OMPA) OF ESCHERICHIA-COLI CAN BE TRANSLOCATED ACROSS THE CYTOPLASMIC MEMBRANE OF BACILLUS-SUBTILIS [J].

MEENS, J ;

FRINGS, E ;

KLOSE, M ;

FREUDL, R .

MOLECULAR MICROBIOLOGY, 1993, 9 (04) :847-855

[10] MUTATIONS IN GENES DOWNSTREAM OF THE RPON GENE (ENCODING SIGMA-54) OF KLEBSIELLA-PNEUMONIAE AFFECT EXPRESSION FROM SIGMA-54-DEPENDENT PROMOTERS [J].

MERRICK, MJ ;

COPPARD, JR .

MOLECULAR MICROBIOLOGY, 1989, 3 (12) :1765-1775

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