VIRAL SPECIFICITY OF HEPATITIS-E VIRUS-ANTIGENS IDENTIFIED BY FLUORESCENT-ANTIBODY ASSAY USING RECOMBINANT HEV PROTEINS

被引：14

作者：

PURDY, MA

CARSON, D

MCCAUSTLAND, KA

BRADLEY, DW

BEACH, MJ

KRAWCZYNSKI, K

机构：

[1] Hepatitis Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

来源：

JOURNAL OF MEDICAL VIROLOGY | 1994年 / 44卷 / 02期

关键词：

WESTERN BLOT; PRIMATE; HEPATOCYTES;

D O I：

10.1002/jmv.1890440217

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A fluorescent antibody (FA) assay for hepatitis E vi rus antigen (HEVAg) in infected liver tissue was used to confirm the presence of virus-specific antigens in hepatocytes during the course of infection. With the cloning of the HEV genome it is now possible to determine which viral antigens are recognized by this FA assay. Recombinant HEV proteins covering the carboxyl half of HEV open reading frame 2 (ORF2) were used in this study to demonstrate that some of the most immunoreactive virus-specific antigens detected by FA are contained within this region of ORF2 (nucleotides 6169-7126). (C) 1994 Wiley-Liss, Inc.

引用

页码：212 / 214

页数：3

共 15 条

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