HOMOLOGOUS RECOMBINATION IN BOVINE PAPILLOMAVIRUS SHUTTLEVECTER - EFFECT OF RELATIVE ORIENTATION OF SUBSTRATE SEQUENCES

被引：2

作者：

NAITO, A

KITAMURA, Y

YOSHIKURA, H

机构：

[1] Department of Bacteriology, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, 113, 7-3-1, Hongo

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)90521-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Relative orientation of recombination substrates, neo gene, strongly influenced homologous recombination events in a bovine papillomavirus shuttle vector. Between direct repeats, recombination occurred at a high frequency while between inverted repeats, it was rare. Double strand break near the mutation site increased the recombination frequency between inverted repeats but not between direct repeats. Formation of long heteroduplex as a recombination intermediate may explain this apparently paradoxical phenomenon. © 1991.

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页码：305 / 312

页数：8

相关论文

共 22 条

[1] ON THE FORMATION OF SPONTANEOUS DELETIONS - THE IMPORTANCE OF SHORT SEQUENCE HOMOLOGIES IN THE GENERATION OF LARGE DELETIONS [J].

ALBERTINI, AM ;

HOFER, M ;

CALOS, MP ;

MILLER, JH .

CELL, 1982, 29 (02) :319-328

[2] REPAIR OF SINGLE-STRANDED-DNA NICKS, GAPS, AND LOOPS IN MAMMALIAN-CELLS [J].

AYARES, D ;

GANEA, D ;

CHEKURI, L ;

CAMPBELL, CR ;

KUCHERLAPATI, R .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (05) :1656-1662

[3]

FEINBERG AP, 1984, ANAL BIOCHEM, V137, P266

[4] EFFICIENT CORRECTION OF MISMATCHED BASES IN PLASMID HETERODUPLEXES INJECTED INTO CULTURED MAMMALIAN-CELL NUCLEI [J].

FOLGER, KR ;

THOMAS, K ;

CAPECCHI, MR .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (01) :70-74

[5]

Gorman C., 1985, DNA CLONING PRACTICA, V1, P143

[6] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

HANAHAN, D .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580

[7] SELECTIVE EXTRACTION OF POLYOMA DNA FROM INFECTED MOUSE CELL CULTURES [J].

HIRT, B .

JOURNAL OF MOLECULAR BIOLOGY, 1967, 26 (02) :365-&

[8] HOMOLOGOUS RECOMBINATION IN A MAMMALIAN PLASMID [J].

KITAMURA, Y ;

YOSHIKURA, H ;

KOBAYASHI, I .

MOLECULAR & GENERAL GENETICS, 1990, 222 (2-3) :185-191

[9] MODEL FOR HOMOLOGOUS RECOMBINATION DURING TRANSFER OF DNA INTO MOUSE L-CELLS - ROLE FOR DNA ENDS IN THE RECOMBINATION PROCESS [J].

LIN, FL ;

SPERLE, K ;

STERNBERG, N .

MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (06) :1020-1034

[10] INTERMOLECULAR RECOMBINATION BETWEEN DNAS INTRODUCED INTO MOUSE L-CELLS IS MEDIATED BY A NONCONSERVATIVE PATHWAY THAT LEADS TO CROSSOVER PRODUCTS [J].

LIN, FLM ;

SPERLE, K ;

STERNBERG, N .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (01) :103-112