ANTHER CULTURE AND HORDEUM BULBOSUM-DERIVED BARLEY DOUBLED HAPLOIDS - MUTATIONS AND METHYLATION

被引：29

作者：

DEVAUX, P

KILIAN, A

KLEINHOFS, A

机构：

[1] WASHINGTON STATE UNIV, DEPT CROP & SOIL SCI, PULLMAN, WA 99164 USA

[2] WASHINGTON STATE UNIV, DEPT GENET & CELL BIOL, PULLMAN, WA 99164 USA

[3] FLORIMOND DESPREZ CO, BIOTECHNOL LAB, F-59242 CAPPELLE PEVALE, FRANCE

[4] SILESIAN UNIV, DEPT GENET, PL-40032 KATOWICE, POLAND

来源：

MOLECULAR AND GENERAL GENETICS | 1993年 / 241卷 / 5-6期

关键词：

ANTHER CULTURE; HORDEUM BULBOSUM METHOD; DOUBLED HAPLOID; MUTATION FREQUENCY; DNA METHYLATION;

D O I：

10.1007/BF00279910

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anther culture and Hordeum bulbosum-derived doubled haploid (DH) lines of barley (Hordeum vulgare L.) were analyzed for RFLP and RAPD polymorphisms. Polymorphisms were not detected in the anther cultureor H. bulbosum-derived DH lines among 273 RFLP and 89 polymerase chain reaction (PCR)-amplified DNA fragments assayed. It was calculated that base substitution or small deletion/insertion mutations had not been induced among 401640 bp screened. Large deletion/insertion mutations were not observed among 33 Mb screened. Polymorphisms were observed when DNA was digested with the methylation-sensitive restriction enzymes HpaII and MspI: these RFLPs originated primarily from the anther culture-derived doubled haploids. The data indicate that heritable DNA methylation changes had occurred during DH production,

引用

页码：674 / 679

页数：6