CONSTRUCTION OF AGROBACTERIUM STRAINS BY ELECTROPORATION OF GENOMIC DNA AND ITS UTILITY IN ANALYSIS OF CHROMOSOMAL VIRULENCE MUTATIONS

被引：14

作者：

CHARLES, TC ^{[1
]}

DOTY, SL ^{[1
]}

NESTER, EW ^{[1
]}

机构：

[1] UNIV WASHINGTON, DEPT MICROBIOL, SEATTLE, WA 98195 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 11期

关键词：

D O I：

10.1128/AEM.60.11.4192-4194.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background but differ in their types of pTi (octopine or nopaline). Both nopaline and octopine pTi-carrying chvE mutants were deficient in vir regulon induction and exhibited similar reductions in host range.

引用

页码：4192 / 4194

页数：3

共 22 条

[11] GENETICS FOR ALL BACTERIA
HOLLOWAY, BW
[J]. ANNUAL REVIEW OF MICROBIOLOGY, 1993, 47 : 659 - 684
[12] A CHROMOSOMAL AGROBACTERIUM-TUMEFACIENS GENE REQUIRED FOR EFFECTIVE PLANT SIGNAL TRANSDUCTION
HUANG, MW
CANGELOSI, GA
HALPERIN, W
NESTER, EW
[J]. JOURNAL OF BACTERIOLOGY, 1990, 172 (04) : 1814 - 1822
[13] KILBANE JJ, 1991, BIOTECHNIQUES, V10, P354
[14] EFFICIENT TRANSFORMATION OF AGROBACTERIUM-TUMEFACIENS BY ELECTROPORATION
MERSEREAU, M
PAZOUR, GJ
DAS, A
[J]. GENE, 1990, 90 (01) : 149 - 151
[15] ELECTROPORATION OF MEGAPLASMIDS INTO AGROBACTERIUM
MOZO, T
HOOYKAAS, PJJ
[J]. PLANT MOLECULAR BIOLOGY, 1991, 16 (05) : 917 - 918
[16] DISTRIBUTION OF 2 AGROBACTERIUM-TUMEFACIENS INSERTION ELEMENTS IN NATURAL ISOLATES - EVIDENCE FOR STABLE ASSOCIATION BETWEEN TI PLASMIDS AND THEIR BACTERIAL HOSTS
PAULUS, F
RIDE, M
OTTEN, L
[J]. MOLECULAR & GENERAL GENETICS, 1989, 219 (1-2): : 145 - 152
[17] VIRA, THE PLANT-SIGNAL RECEPTOR, IS RESPONSIBLE FOR THE TI PLASMID-SPECIFIC TRANSFER OF DNA TO MAIZE BY AGROBACTERIUM
RAINERI, DM
BOULTON, MI
DAVIES, JW
NESTER, EW
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (08) : 3549 - 3553
[18] EFFICIENT TRANSFORMATION OF AGROBACTERIUM SPP BY HIGH-VOLTAGE ELECTROPORATION
SHEN, WJ
FORDE, BG
[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (20) : 8385 - 8385
[19] CONTROL OF EXPRESSION OF AGROBACTERIUM-VIR GENES BY SYNERGISTIC ACTIONS OF PHENOLIC SIGNAL MOLECULES AND MONOSACCHARIDES
SHIMODA, N
TOYODAYAMAMOTO, A
NAGAMINE, J
USAMI, S
KATAYAMA, M
SAKAGAMI, Y
MACHIDA, Y
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (17) : 6684 - 6688
[20] VIRA AND VIRG CONTROL THE PLANT-INDUCED ACTIVATION OF THE T-DNA TRANSFER PROCESS OF A-TUMEFACIENS
STACHEL, SE
ZAMBRYSKI, PC
[J]. CELL, 1986, 46 (03) : 325 - 333

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