A Rapid DNA Extraction Method for Sugarcane and its Relatives

被引：39

作者：

Honeycutt, Rhonda J. ^{[1
,2
]}

Sobral, Bruno W. S. ^{[2
]}

Keim, Paul ^{[3
]}

Irvine, James E. ^{[1
]}

机构：

[1] Texas A&M Univ, Agr Res & Extens Ctr, Weslaco, TX 78596 USA

[2] Calif Inst Biol Res, La Jolla, CA 92037 USA

[3] No Arizona Univ, Flagstaff, AZ 86011 USA

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1992年 / 10卷 / 01期

关键词：

DNA extraction; Saccharum complex; grasses; sugarcane; Andropogoneae;

D O I：

10.1007/BF02669266

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple DNA extraction method based on CTAB precipitation was used to obtain DNA from members of the genus Saccharum and related species. DNA yields and purities were similar for all Saccharum species sampled. The method described here resulted in high quality total DNA suitable for polymerase chain reaction (PCR)-based techniques as well as restriction endonuclease digestion, Southern hybridization, and DNA cycle-sequencing.

引用

页码：66 / 72

页数：7