IDENTIFICATION OF A LIGAND FOR THE C-KIT PROTOONCOGENE

被引：995

作者：

WILLIAMS, DE

EISENMAN, J

BAIRD, A

RAUCH, C

VANNESS, K

MARCH, CJ

PARK, LS

MARTIN, U

MOCHIZUKI, DY

BOSWELL, HS

BURGESS, GS

COSMAN, D

LYMAN, SD

机构：

[1] IMMUNEX CORP,DEPT PROT CHEM,SEATTLE,WA 98101

[2] IMMUNEX CORP,DEPT MOLEC BIOL,SEATTLE,WA 98101

[3] IMMUNEX CORP,DEPT BIOCHEM,SEATTLE,WA 98101

[4] INDIANA UNIV,VET ADM HOSP,SCH MED,DEPT MED HEMATOL ONCOL,INDIANAPOLIS,IN 46223

来源：

CELL | 1990年 / 63卷 / 01期

关键词：

D O I：

10.1016/0092-8674(90)90297-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proilferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit proto-oncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein. © 1990.

引用

页码：167 / 174

页数：8

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