CONVENIENT METHOD TO PURIFY HEMOGLOBIN

被引：9

作者：

SAKAI, H

TAKEOKA, S

NISHIDE, H

TSUCHIDA, E

机构：

[1] Department of Polymer Chemistry, Waseda University, Tokyo

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY | 1994年 / 22卷 / 03期

关键词：

D O I：

10.3109/10731199409117895

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A convenient method to purify Hb solution from outdated RBC has been established for the starting material of Hb-based blood substitutes. To prevent MetHb formation during the procedure, Hb in RBC was carbonylated in advance. Then RBC was mixed with organic solvent for hemolysis and centrifuged for removal of stroma. The resulting SFHb solution was heated at 60 degrees C and generated precipitates were removed out by centrifugation. The purity of Hb (25 g/dl) was confirmed by SDS-PAGE. IEF and oxygen binding property of the Kb solution also guaranteed its purity and no denaturation of Hb. This method is applicable to large scale production of the purified Hb for the starting material of Hb-based blood substitutes.

引用

页码：651 / 656

页数：6

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