RECOVERY AND MEASUREMENT OF VOLATILES FROM LIPIDS HYDROCARBONS IN IRRADIATED FATS

Techniques are described for the isolation and quantitative measurement of a wide range of volatile compounds in lipids. The higher-boiling compounds are collected by cold-finger vacuum distillation and their quantities in the fat are determined by relating their gas chromatographic peak areas to that of an appropriate internal standard. The lower-boiling compounds are recovered on a short precolumn packed with stainless steel helices, which can be fitted inside the gas chromatographic oven before the inlet of an alumina column. Recovery data are given for a series of hydrocarbons from Cj to C22. The methods described in this report were used mainly for the measurement of hydrocarbons in a number of irradiated fats, but can also be applied for the analysis of other classes of compounds. © 1969, American Chemical Society. All rights reserved.