MAGAININS AFFECT RESPIRATORY CONTROL, MEMBRANE-POTENTIAL AND MOTILITY OF HAMSTER SPERMATOZOA

被引：41

作者：

DEWAAL, A

GOMES, AV

MENSINK, A

GROOTEGOED, JA

WESTERHOFF, HV

机构：

[1] NETHERLANDS CANC INST,MOLEC BIOL SECT,H5,PLESMANLAAN 121,1066 CX AMSTERDAM,NETHERLANDS

[2] ERASMUS UNIV,DEPT ENDOCRINOL & REPROD,3000 DR ROTTERDAM,NETHERLANDS

[3] UNIV AMSTERDAM,EC SLATER INST BIOCHEM RES,1018 TV AMSTERDAM,NETHERLANDS

来源：

FEBS LETTERS | 1991年 / 293卷 / 1-2期

关键词：

MAGAININ; SPERMICIDAL; SPERM MOTILITY; SPERMATID; ENERGETICS; PEPTIDE-BASED DEFENSE;

D O I：

10.1016/0014-5793(91)81191-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hypothesis was tested that the magainin peptides, known to compromise bacterial and mitochondrial energetics, are highly active against spermatozoa. A mixture of magainin A and PGLa (1:1) caused a 50% reduction in motility of hamster spermatozoa at 4-mu-g/ml total peptide concentration. All motility was lost at 8-mu-g/ml. At this concentration. respiratory control was released and respiration in the presence of uncoupler was inhibited. Uptake of the lipophilic cation tetraphenyl phosphonium was largely abolished by addition of magainin A and PGLa at this concentration, indicative of a decrease in the mitochondrial membrane potential. Magainin A and PGLa showed synergism with respect to release of respiratory control.

引用

页码：219 / 223

页数：5

共 33 条

[21] MEMBRANE-POTENTIAL OF MITOCHONDRIA MEASURED WITH AN ELECTRODE SENSITIVE TO TETRAPHENYL PHOSPHONIUM AND RELATIONSHIP BETWEEN PROTON ELECTROCHEMICAL POTENTIAL AND PHOSPHORYLATION POTENTIAL IN STEADY-STATE [J].