STUDIES ON THE ENZYMATIC-HYDROLYSIS OF BOUND AROMA CONSTITUENTS FROM RASPBERRY FRUIT PULP

HRGC and HRGC-MS identifications of bound volatiles from raspberry fruit (Rubus idaeus cv. Heritage) pulp were achieved after isolation of an extract obtained by Amberlite XAD-2 adsorption and methanol elution followed by hydrolysis with a commercial pectinase enzyme. In total, 57 bound aglycons were identified for the first time in raspberry fruit originating from fatty acid, phenylpropanoid, and terpene metabolisms. Among them, the following C-13 norisoprenoids were detected: 3-hydroxy-alpha-ionone, 3-hydroxy-beta-ionone, 3-hydroxy-5,6-epoxy-beta-ionone (tent.), 4-hydroxy-beta-ionone, 3,4-didehydro-beta-ionone, alpha-ionol, 3-oxo-alpha-ionol, 3-oxo-7,8-dihydro-alpha-ionol, 4-oxo-beta-ionol, and 3-hydroxy-beta-damascone.