7,8-DIHYDRO-8-OXOADENINE AS A REPLACEMENT FOR CYTOSINE IN THE 3RD STRAND OF TRIPLE HELICES - TRIPLEX FORMATION WITHOUT HYPOCHROMICITY

被引：70

作者：

JETTER, MC ^{[1
]}

HOBBS, FW ^{[1
]}

机构：

[1] DUPONT MERCK PHARMACEUT CO, POB 80328, WILMINGTON, DE 19880 USA

来源：

BIOCHEMISTRY | 1993年 / 32卷 / 13期

关键词：

D O I：

10.1021/bi00064a006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligonucleotides containing thymine and cytosine (or 5-methylcytosine) bases are known to bind to specific homopurine sequences in double-stranded DNA by means of T.AT and C+.GC base triplets. Cytosine in the third strand of such triple helices can be completely replaced by 7,8-dihydro-8-oxoadenine, a base which should not require protonation to form base triplets. Experiments using native PAGE and inhibition of triplex-directed photo-cross-linking demonstrate that triplexes with 7,8-dihydro-8-oxoadenine in the third strand are as stable at pH 6.0 as triplexes with 5-methylcytosine. The stability of triplexes with 7,8-dihydro-8-oxoadenine, unlike those with 5-methylcytosine, is not substantially diminished upon raising the pH to 7.4. Surprisingly, triplex formation with an oligonucleotide containing only thymine and 7,8-dihydro-8-oxoadenine was not associated with significant hypochromicity and could not be detected in conventional thermal denaturation experiments.

引用

页码：3249 / 3254

页数：6

共 44 条

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