学术探索
学术期刊
新闻热点
数据分析
智能评审

EXPRESSION OF HUMAN LYSOSOMAL BETA-HEXOSAMINIDASE IN YEAST VACUOLES

被引：5
作者：
PREZANT, TR
机构：
[1] Department of Biological Chemistry, UCLA School of Medicine, Los Angeles
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 01期
关键词：
D O I：
10.1016/0006-291X(90)91285-Z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The yeast Saccharomyces cerevisiae was tested as a recipient for functional expression of a mammalian lysosomal enzyme. The β chain of human β-hexosaminidase formed active dimeric enzyme, HexB, in transformants. HexB activity was localized to the vacuole, the yeast counterpart to the lysosome. A simple in situ enzyme assay was developed, which could be useful in expressing other lysosomal cDNAs. © 1990.
引用
收藏
页码：383 / 390
页数：8
相关论文
共 31 条
1234