EVALUATION OF O-PHTHALALDEHYDE AS BIFUNCTIONAL FLUOROGENIC POSTCOLUMN REAGENT FOR GLUTATHIONE IN LC

被引：45

作者：

LEROY, P

NICOLAS, A

WELLMANN, M

MICHELET, F

OSTER, T

SIEST, G

机构：

[1] FAC SCI PHARMACEUT & BIOL NANCY,CHIM ANALYT & BROMATOL LAB,CNRS,URA 597,BP 403,F-54001 NANCY,FRANCE

[2] FAC SCI PHARMACEUT & BIOL NANCY,CTR MEDICAMENT,CNRS,URA 597,F-54001 NANCY,FRANCE

来源：

CHROMATOGRAPHIA | 1993年 / 36卷

关键词：

COLUMN LIQUID CHROMATOGRAPHY; GLUTATHIONE; O-PHTHALALDEHYDE; POSTCOLUMN DERIVATIZATION; PLASMA;

D O I：

10.1007/BF02263849

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the assay of various forms of glutathione in human plasma has been developed. The reduced form was measured after direct injection of deproteinized plasma with perchloric acid. The unbound oxidized forms were assayed in deproteinized plasma after reduction with dithiothreitol. The total amount of glutathione was determined by reduction before protein precipitation. Separation of reduced glutathione from other endogenous thiols was by ion-pair, reversed-phase, high-performance liquid chromatography. Post-column derivatization with ortho-phthalaldehyde made the assay more selective than when using pyrenemaleimide as thiol fluorogepic reagent. The linear range was 0.02 to 2.0 mug mL-1 with good repeatability (relative standard deviation less than 5 % for the lowest concentration measured).

引用

页码：130 / 134

页数：5

共 18 条

[11] REACTION OF O-PHTHALALDEHYDE WITH AMINO-ACIDS AND GLUTATHIONE - APPLICATION TO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY DETERMINATION [J].