THERMOINACTIVATION OF CELLOBIOHYDROLASE-I FROM TRICHODERMA-REESEI QM-9414

被引：14

作者：

JIMENEZ, J ^{[1
]}

DOMINGUEZ, JM ^{[1
]}

CASTILLON, MP ^{[1
]}

ACEBAL, C ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC CIENCIAS BIOL,DEPT BIOQUIM & BIOL MOLEC 1,E-28040 MADRID,SPAIN

来源：

CARBOHYDRATE RESEARCH | 1995年 / 268卷 / 02期

关键词：

CELLULASE; CELLOBIOHYDROLASE I; THERMAL INACTIVATION; TRICHODERMA REESEI;

D O I：

10.1016/0008-6215(94)00337-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Irreversible thermoinactivation of cellobiohydrolase I from Trichoderma reesei has been analyzed at 70 degrees C and pH 4.8. The time course of thermal inactivation and the dependence of the inactivation rates on protein concentration suggested that aggregation followed by precipitation was the main process leading to irreversible thermoinactivation. The enzyme activity was very resistant to 4 M urea which stabilized the enzyme against thermal inactivation. Deamidation of Asn/Gln residues and hydrolysis of peptide bonds were responsible for the loss of enzyme activity at long times of exposure at 70 degrees C.

引用

页码：257 / 266

页数：10

共 25 条

[21] SEPARATION OF ENDO-TYPE AND EXO-TYPE CELLULASES USING A NEW AFFINITY-CHROMATOGRAPHY METHOD [J].

VANTILBEURGH, H ;

BHIKHABHAI, R ;

PETTERSSON, LG ;

CLAEYSSENS, M .

FEBS LETTERS, 1984, 169 (02) :215-218

[22]

WHITAKER JR, 1980, CHEM DETERIORIATION, P1

[23] THE COMPETITIVE-INHIBITION OF TRICHODERMA-REESEI C30 CELLOBIOHYDROLASE-I BY GUANIDINE-HYDROCHLORIDE [J].

WOODWARD, J ;

CARMICHAEL, JS ;

CAPPS, KM ;

HERRMANN, PC ;

LEE, NE .

FEBS LETTERS, 1990, 270 (1-2) :143-146

[24] ON THE ROLE OF REVERSIBLE DENATURATION (UNFOLDING) IN THE IRREVERSIBLE THERMAL INACTIVATION OF ENZYMES [J].

ZALE, SE ;

KLIBANOV, AM .

BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (09) :2221-2230

[25] WHY DOES RIBONUCLEASE IRREVERSIBLY INACTIVATE AT HIGH-TEMPERATURES [J].

ZALE, SE ;

KLIBANOV, AM .

BIOCHEMISTRY, 1986, 25 (19) :5432-5444

← 1 2 3 →